| Solution Information | help | |
| Enzyme: | Type-1 angiotensin II receptor | |
| inhibitor: | BDBM76119 | |
| substrate: | n/a | |
| Solution Type: | Aqueous | |
| pH at Preparation: | n/a | |
| Temp. Prep.: | n/a | |
| Comments: | A. Brief Description of the Assay: The purpose of this assay is to detect agonists that can activate the angiotensin II receptor type 1 (AT1) in the CHO-K1 AGTR-1 b-Arrestin Cell Line in 1536-well plate format in HTS mode. B. Materials: Angiotensin II receptor type 1 (AGTR-1) Cell Line (DiscoveRx, Cat# 93-0312C2) F12 nutrient mix HAMs (Invitrogen, Cat# 11765) Fetal Bovine Serum, heat-inactivated (Hyclone, Cat# SH30396) 100X Penicillin/Streptomycin Solution (Invitrogen, Cat#15140-122) Hygromycin B (Roche, Cat# 10843555001) Geneticin (MPBiomedicals, Cat # 1672548) Trypsin-EDTA 0.25% (Invitrogen, Cat# 25200-056) Cell Dissociation Buffer (Invitrogen, Cat # 13151) DPBS (Hyclone, Cat# 30028.02) T225 TC Flask (Nunc, Cat# 159934) Cell strainer, 40 um (BD, Cat# 352340) 1536-well, white, solid-bottom, Kalypsys compatible, TC plate (Corning) Angiotensin II (Sigma-Aldrich, Cat # A9525) PathHunter Detection Reagents (DiscoveRx, Cat# 93-0001) Galacton Star Emerald 11 Cell Assay Buffer C. uHTS P | |
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